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A similar K m value of 0.25 mM was reported by Garca-Canovas and co-workers for Agaricus bisporus tyrosinase 26

Animal studies using collagen tubes containing GHK-Cu showed increased nerve fiber regeneration, higher axon counts, and greater Schwann cell proliferation

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After incubation with the secondary antibody, the cells were washed with PBST and mounted using a Fluoroshield Mounting Medium with DAPI (Abcam ab104139)